![]() ![]() However, antibiotic residues can be found at higher levels in wastewater or surface waters with concentrations ranging from 1 ng/L to 1 µg/L, which is considered a public health problem. Thus, the European Union has therefore set the maximum tolerance limit for the presence of AMPs in certain foods at 50 µg/kg. This pollution has numerous impacts on human health, such as epileptic seizures and allergic reactions. ![]() The presence of MDR bacteria has been found in treated wastewater effluents, with the most common antibiotic resistance being ampicillin (83.3%). The frequent use of AMP as an antibiotic has led to the emergence of multi-drug resistant (MDR) bacteria that threaten human health. It is classified as a broad-spectrum antibiotic, so its use is common in both humans and animals, resulting in the presence of residual amounts in the environment. ![]() This biosensor can be considered a promising device for the detection of antibiotics in the environment as a point-of-use system.Īmpicillin (AMP) is a β-lactam antibiotic widely used as a medication for bacterial infections such as pneumonia, bronchitis, and urinary tract infections. A high selectivity towards other contaminants was demonstrated with this biosensor and the analysis of real river water samples without any treatment showed good recovery results thanks to the antifouling properties. An amperometric variation of the naphthoquinone response was obtained regarding the ampicillin concentration with a limit of detection (LOD) of 10 pg/L (0.28 pM). Thus, a highly sensitive redox process was obtained that could follow the recognition process between the apatamer and the target. We demonstrated that the nanocomposite formed with PPyNPs and MoS2 nanosheets has electro-catalytic properties and conductivity leading to a synergetic effect on the electrochemical redox process of the redox marker. Cyclic voltammetry and electrochemical impedance spectroscopy were used to analyze their electrocatalytic properties. High-resolution XPS measurements demonstrated the formation of a binding between the two nanomaterials and energy transfer affording the formation of heterostructure. The structural and morphological properties of the nanocomposite were studied by SEM, AFM, and FT-IR. Fine chemistry is developed to build the biosensors entirely based on robust covalent immobilizations of naphthoquinone as a redox marker and the aptamer. We studied the transducing potential of nanostructure composed of MoS2 nanosheets and conductive polypyrrole nanoparticles (PPyNPs) cast on a screen-printed electrode. We report the design of an electrochemical aptasensor for ampicillin detection, which is an antibiotic widely used in agriculture and considered to be a water contaminant. ![]()
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